A master's thesis was discussed at Anbar University - College of Science and tagged (Development of composite – gold nanoparticle hybrid materials for biochemical applications).

    The aim of the thesis was submitted by the student (Mahmoud Khaled Bura') from the Department of Physics the cold atmospheric plasma has been used in the synthesis of gold nanoparticles of Aqueous gold tetrachloride salts HAuCl4.4H2O mixed with Hyaluronic acid (HA) using a low-cost and simple method in order to create a cold plasma.

    A group of samples was examined to study the best concentration of gold salt, as well as the best exposure time for plasma, in addition, to study the effect of mixing with different concentrations ratio to find the best mixing ratio of gold salt and Hyaluronic acid (HA).

     X-ray diffraction (XRD), UV-Visible spectra were used to characterize the nanoparticles. The synthesis of AuNPs indicated surface Plasmon resonance (SPR) at 530, 540, and 550 nm for samples that were prepared. The strong intense peaks that showed in the XRD patterns for all prepared samples is indicating to the face-centered cubic structure and nature of crystalline of nanoparticles. The average size of crystallite was from 17 to 36 nm for the AuNPs, and the FESEM showed the AuNPs morphology. And the synthesize of AuNPs were showed by observations are have a spherical shape sizes from 4 to 60 nm. And using DPPH to check antioxidant activity for gold nanoparticles and the mixture of gold nanoparticles with Hyaluronic acid (HA ).

    The outcome of the study revealed that the cold atmospheric plasma is a promising technique to be used in the production of the nanoparticle materials for medical application.